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KMID : 0364219880310030236
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Korean Journal of Zoology
1988 Volume.31 No. 3 p.236 ~ p.242
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A Study on the Speciadon of a Fresh Water Fish Zacco temminckL VII. Vadation of Mitochondrial DNA between 2 Types of Zacco temmincki
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Lee Hei-Yung
Yang Suh-Yung
Park Chang-Shin
Yu Sung-Lim
Lee Sung-Keun
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Abstract
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Mitochondrial DNAs of two Mdh allelotypes of the dark chub, Z. temmincki inhabiting in Korean fresh water, were analysed. Samples of each type were collected from four populations, and the fragment patterns for mtdNA of each type were explained from 7 of the eleven restriction enzymes with hexanucleotide recognition site. Genome size was approximately 16.7 kilobases. The highly typical mtdNA fragments of each type were discovered in digestion profiles produced by Eco RI and Pst I enzyrnes. The comparisons of restriction fragment patterns and relative digestion maps permitted the estimation of fragment homology (F) and nucleotide sequence divergence(p). Between the two identical types, sequence divergence(p) was 0.128(MS), and 0.045(MM), ; between the two different types, 0.195 (range 0.177-0.226). These result may provide a distinct difference more than the value derived from allozyrne analysis, and a powerful new molecular approach for assessing genetic-evolutionary relationship among fishes.
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KEYWORD
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Zacco temmincki , Speciation , Mitochondrial DNA
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